Mário Gadanho of SGS Molecular speaks of how subsequent technology sequencing could possibly be an important technique of routinely detecting meals fraud. With over 20 years’ expertise in DNA analysis, Mário Gadanho spoke at Meals Fraud 2019 about how subsequent technology sequencing (NGS) could possibly be used to determine the content material of a overwhelming majority of meals, equivalent to fish, meat, vegetation, microbes, probiotics and microorganisms. He described how the method presently being developed is an untargeted method, utilizing DNA sequencing, and described his lab to be one of many first to make use of NGS for meals authenticity.Actual-time PCR is usually the method used for meals ingredient identification, nonetheless tends to be a focused method, that means that scientists must enter parameters. With NGS being an untargeted method, quite a few DNA samples might be sequenced and concurrently run to determine the species of various organic samples, on the identical time. Giving the instance of fish, Mário defined how ‘the extra processed a product is, the harder it’s to determine’. The species of freshly caught fish might be simply recognized, and while the species of a fillet of fish is barely tougher to find out, and that of a block is extraordinarily troublesome, to determine the origin of fish powder just by it’s near not possible. With NGS, a reasonably quick process might decide the contents of the powder, leading to particulars displaying the content material of all species within the pattern.The primary limitation described of the method, was that for very processed meals, it could possibly be troublesome to extract DNA, and so solely quick fragments of DNA could also be obtained.NGS consists of 4 foremost steps. Initially, DNA would should be extracted from the pattern, which, relying on the product and the way processed it’s, might take as much as 16 hours. The following step is getting ready the DNA library, the place various samples from completely different merchandise might be ready, usually taking round four hours. Then, sequencing of those DNA samples would happen, which might lead to a FASTQ file, containing the sequenced DNA.At this level, the FASTQ file might be uploaded to varied databases, some publicly obtainable, and bioinformatic analyses might decide the precise species in every organic pattern. These computational analyses might be very fast to find out species, nonetheless if various samples are being examined on the identical time, it’s going to take longer to obtain outcomes.As various samples might be run concurrently, there’s a big discount in value.With the system being automated, Mário added that it’s ‘particular, delicate and dependable.’